| Date | Home | Away | Result |
|---|---|---|---|
| 4/9/21 1:45 PM | Watford FC | Reading FC | 2-0 |
| 4/10/21 6:30 AM | Millwall FC | Swansea City | 0-3 |
| 4/10/21 9:00 AM | Preston North End | Brentford FC | 0-5 |
| 4/10/21 9:00 AM | Wycombe Wanderers | Luton Town | 1-3 |
| 4/10/21 9:00 AM | Bristol City | Nottingham Forest | 0-0 |
| 4/10/21 9:00 AM | Barnsley FC | Middlesbrough FC | 2-0 |
| 4/10/21 9:00 AM | AFC Bournemouth | Coventry City | 4-1 |
| 4/10/21 9:00 AM | Huddersfield Town | Rotherham United | 0-0 |
| 4/10/21 9:00 AM | Birmingham City | Stoke City | 2-0 |
| 4/10/21 9:00 AM | Derby County | Norwich City | 0-1 |
| 4/10/21 9:00 AM | Cardiff City | Blackburn Rovers | 2-2 |
| 4/10/21 9:00 AM | Queens Park Rangers | Sheffield Wednesday | 4-1 |