| Date | Home | Away | Result |
|---|---|---|---|
| 5/15/16 8:30 AM | SC Paderborn 07 | 1. FC Nürnberg | 0-1 |
| 5/15/16 8:30 AM | Eintracht Braunschweig | Fortuna Düsseldorf | 0-2 |
| 5/15/16 8:30 AM | FSV Frankfurt | TSV 1860 Munich | 2-1 |
| 5/15/16 8:30 AM | 1. FC Union Berlin | SC Freiburg | 2-1 |
| 5/15/16 8:30 AM | Karlsruher SC | Arminia Bielefeld | 1-1 |
| 5/15/16 8:30 AM | SpVgg Greuther Fürth | SV Sandhausen | 3-1 |
| 5/15/16 8:30 AM | MSV Duisburg | RB Leipzig | 1-0 |
| 5/15/16 8:30 AM | 1. FC Heidenheim 1846 | VfL Bochum | 2-4 |
| 5/15/16 8:30 AM | FC St. Pauli | 1. FC Kaiserslautern | 5-2 |