| Date | Home | Away | Result |
|---|---|---|---|
| 11/3/23 2:45 PM | Barnsley FC | Horsham FC | 3-3 |
| 11/3/23 2:45 PM | Sheppey United | FC Walsall | 1-4 |
| 11/4/23 8:30 AM | Northampton Town | Barrow FC | 1-3 |
| 11/4/23 10:00 AM | Leyton Orient | Carlisle United | 3-1 |
| 11/4/23 10:00 AM | Bolton Wanderers | Solihull Moors FC | 4-0 |
| 11/4/23 10:00 AM | Exeter City | Wigan Athletic | 0-2 |
| 11/4/23 10:00 AM | Oxford United | Maidenhead Utd | 2-0 |
| 11/4/23 10:00 AM | Newport County | Oldham Athletic | 2-0 |
| 11/4/23 10:00 AM | Hereford FC | FC Gillingham | 0-2 |
| 11/4/23 10:00 AM | Marine AFC | Harrogate Town FC | 1-5 |
| 11/4/23 10:00 AM | Peterborough United | Salford City | 2-2 |
| 11/4/23 10:00 AM | Eastleigh FC | Boreham Wood | 5-1 |
| 11/4/23 10:00 AM | FC Port Vale | Burton Albion | 0-0 |
| 11/4/23 10:00 AM | Lincoln City | FC Morecambe | 1-2 |
| 11/4/23 10:00 AM | Sutton United | AFC Fylde | 2-1 |
| 11/4/23 10:00 AM | Bradford City | Wycombe Wanderers | 1-2 |
| 11/4/23 10:00 AM | Shrewsbury Town | Colchester United | 3-2 |
| 11/4/23 10:00 AM | Notts County | Crawley Town | 3-2 |
| 11/4/23 10:00 AM | Reading FC | Milton Keynes Dons FC | 3-2 |
| 11/4/23 10:00 AM | Doncaster Rovers | Accrington Stanley | 2-2 |
| 11/4/23 10:00 AM | AFC Wimbledon | Cheltenham Town | 5-1 |
| 11/4/23 10:00 AM | Yeovil Town | FC Gateshead | 3-2 |
| 11/4/23 10:00 AM | Stevenage FC | Tranmere Rovers | 4-3 |
| 11/4/23 10:00 AM | Cambridge United | Bracknell Town | 2-1 |
| 11/4/23 10:00 AM | Bristol Rovers | Whitby | 7-2 |
| 11/4/23 10:00 AM | Chester FC | York City | 0-0 |
| 11/4/23 10:00 AM | Ramsgate | Woking FC | 2-1 |
| 11/4/23 10:00 AM | Scarborough Athletic | Forest Green Rovers | 1-1 |
| 11/4/23 10:00 AM | Chesham United | Maidstone United | 0-2 |
| 11/4/23 10:00 AM | Alfreton Town FC | Worthing | 2-0 |
| 11/4/23 10:00 AM | Swindon Town | Aldershot Town | 4-7 |
| 11/4/23 10:00 AM | Stockport County | Worksop | 5-1 |
| 11/4/23 10:00 AM | Curzon Ashton FC | FC Barnet | 0-1 |
| 11/4/23 12:45 PM | Bromley FC | FC Blackpool | 0-2 |
| 11/4/23 2:45 PM | Mansfield Town | AFC Wrexham | 1-2 |
| 11/5/23 6:15 AM | FC Chesterfield | FC Portsmouth | 1-0 |
| 11/5/23 8:00 AM | Kidderminster Harriers | Fleetwood Town | 1-2 |
| 11/5/23 8:00 AM | Slough Town FC | Grimsby Town | 1-1 |
| 11/5/23 8:45 AM | Crewe Alexandra | Derby County | 2-2 |
| 11/5/23 11:30 AM | Charlton Athletic | Cray Valley Paper Mills FC | 1-1 |
| 11/14/23 1:30 PM | Horsham FC | Barnsley FC | 0-3 |
| 11/14/23 1:45 PM | Grimsby Town | Slough Town FC | 7-2 |
| 11/14/23 1:45 PM | Derby County | Crewe Alexandra | 1-3 |
| 11/14/23 1:45 PM | York City | Chester FC | 2-1 |
| 11/14/23 1:45 PM | Burton Albion | FC Port Vale | 0-2 |
| 11/14/23 1:45 PM | Salford City | Peterborough United | 8-9a.PSO |
| 11/14/23 1:45 PM | Accrington Stanley | Doncaster Rovers | 1-2a.e.t |
| 11/14/23 1:45 PM | Forest Green Rovers | Scarborough Athletic | 5-2 |
| 11/15/23 1:45 PM | Cray Valley Paper Mills FC | Charlton Athletic | 1-6 |
| 12/12/23 1:45 PM | Scarborough Athletic | Forest Green Rovers | 2-4 |