| Date | Home | Away | Result |
|---|---|---|---|
| 11/26/22 6:45 AM | Kings Lynn FC | Stevenage FC | 0-3 |
| 11/26/22 9:00 AM | Forest Green Rovers | Alvechurch | 2-1 |
| 11/26/22 9:00 AM | Sheffield Wednesday | Mansfield Town | 2-1 |
| 11/26/22 9:00 AM | FC Walsall | Carlisle United | 2-1 |
| 11/26/22 9:00 AM | FC Portsmouth | Milton Keynes Dons FC | 3-2 |
| 11/26/22 9:00 AM | Barnsley FC | Crewe Alexandra | 3-0 |
| 11/26/22 9:00 AM | Charlton Athletic | Stockport County | 2-2 |
| 11/26/22 9:00 AM | Accrington Stanley | FC Barnet | 1-0 |
| 11/26/22 9:00 AM | Hartlepool United | Harrogate Town FC | 3-1 |
| 11/26/22 9:00 AM | AFC Wimbledon | FC Chesterfield | 0-2 |
| 11/26/22 9:00 AM | Cambridge United | Grimsby Town | 1-2 |
| 11/26/22 9:00 AM | Oxford United | Exeter City | 4-1 |
| 11/26/22 9:00 AM | Shrewsbury Town | Peterborough United | 3-1 |
| 11/26/22 9:15 AM | AFC Wrexham | Farnborough | 4-1 |
| 11/26/22 11:00 AM | Dagenham and Redbridge | FC Gillingham | 1-1 |
| 11/27/22 6:30 AM | Ebbsfleet United | Fleetwood Town | 0-1 |
| 11/27/22 8:00 AM | Bristol Rovers | Boreham Wood | 0-2 |
| 11/27/22 8:00 AM | Burton Albion | Chippenham Town | 6-1 |
| 11/27/22 9:15 AM | Newport County | Derby County | 1-2 |
| 11/27/22 11:00 AM | Ipswich Town | Buxton FC | 4-0 |
| 12/7/22 1:45 PM | Stockport County | Charlton Athletic | 3-1 |
| 12/8/22 1:15 PM | FC Gillingham | Dagenham and Redbridge | 3-2 |