| Date | Home | Away | Result |
|---|---|---|---|
| 9/26/25 1:30 PM | US Catanzaro 1929 | Juve Stabia | 2-2 |
| 9/27/25 8:00 AM | Como Calcio | US Cremonese | 1-1 |
| 9/27/25 8:00 AM | US Avellino | Virtus Entella | 2-0 |
| 9/27/25 8:00 AM | Mantova 1911 | Frosinone Calcio | 1-5 |
| 9/27/25 8:00 AM | FC Cesena | Palermo FC | 1-1 |
| 9/27/25 8:00 AM | FC Südtirol | AC Reggiana 1919 | 3-1 |
| 9/27/25 8:00 AM | FC Venice | Spezia Calcio | 2-0 |
| 9/27/25 10:15 AM | AC Monza | Padova Calcio | 0-1 |
| 9/27/25 11:00 AM | Juventus Turin | Atalanta Bergamo | 1-1 |
| 9/27/25 12:30 PM | SSC Bari | Sampdoria Genua | 1-1 |
| 9/27/25 1:45 PM | Cagliari Calcio | Inter Milan | 0-2 |
| 9/28/25 5:30 AM | Sassuolo Calcio | Udinese Calcio | 3-1 |
| 9/28/25 8:00 AM | Pisa Sporting Club | Fiorentina | 0-0 |
| 9/28/25 8:00 AM | AS Roma | Hellas Verona | 2-0 |
| 9/28/25 10:15 AM | Modena FC | Pescara Calcio | 2-1 |
| 9/28/25 11:00 AM | US Lecce | FC Bologna | 2-2 |
| 9/28/25 12:30 PM | FC Empoli | Carrarese Calcio 1908 | 2-2 |
| 9/28/25 1:45 PM | AC Milan | SSC Napoli | 2-1 |
| 9/29/25 11:30 AM | Parma Calcio 1913 | FC Turin | 2-1 |
| 9/29/25 1:45 PM | Genua CFC | Lazio Rom | 0-3 |