| Date | Home | Away | Result |
|---|---|---|---|
| 9/19/25 12:00 PM | Frosinone Calcio | FC Südtirol | 2-2 |
| 9/19/25 1:45 PM | US Lecce | Cagliari Calcio | 1-2 |
| 9/19/25 2:00 PM | Palermo FC | SSC Bari | 2-0 |
| 9/20/25 8:00 AM | FC Bologna | Genua CFC | 2-1 |
| 9/20/25 8:00 AM | Spezia Calcio | Juve Stabia | 1-3 |
| 9/20/25 8:00 AM | FC Venice | FC Cesena | 1-2 |
| 9/20/25 8:00 AM | AC Reggiana 1919 | US Catanzaro 1929 | 2-2 |
| 9/20/25 10:15 AM | AC Monza | Sampdoria Genua | 1-0 |
| 9/20/25 11:00 AM | Hellas Verona | Juventus Turin | 1-1 |
| 9/20/25 12:30 PM | Mantova 1911 | Modena FC | 1-3 |
| 9/20/25 1:45 PM | Udinese Calcio | AC Milan | 0-3 |
| 9/21/25 5:30 AM | Lazio Rom | AS Roma | 0-1 |
| 9/21/25 8:00 AM | US Cremonese | Parma Calcio 1913 | 0-0 |
| 9/21/25 8:00 AM | FC Turin | Atalanta Bergamo | 0-3 |
| 9/21/25 8:00 AM | Carrarese Calcio 1908 | US Avellino | 3-4 |
| 9/21/25 10:15 AM | Pescara Calcio | FC Empoli | 4-0 |
| 9/21/25 11:00 AM | Fiorentina | Como Calcio | 1-2 |
| 9/21/25 12:30 PM | Padova Calcio | Virtus Entella | 2-1 |
| 9/21/25 1:45 PM | Inter Milan | Sassuolo Calcio | 2-1 |
| 9/22/25 1:45 PM | SSC Napoli | Pisa Sporting Club | 3-2 |