| Date | Home | Away | Result |
|---|---|---|---|
| 10/21/23 5:00 PM | Charlotte FC | Inter Miami CF | 1-0 |
| 10/21/23 5:00 PM | New England Revolution | Philadelphia Union | 2-1 |
| 10/21/23 5:00 PM | New York City FC | Chicago Fire | 1-0 |
| 10/21/23 5:00 PM | Nashville SC | New York Red Bulls | 0-1 |
| 10/21/23 5:00 PM | Cincinnati FC | Atlanta United FC | 2-2 |
| 10/21/23 5:00 PM | Toronto FC | Orlando City | 0-2 |
| 10/21/23 5:00 PM | Columbus Crew | CF Montreal | 2-1 |
| 10/21/23 8:00 PM | Vancouver Whitecaps | Los Angeles FC | 1-1 |
| 10/21/23 8:00 PM | St. Louis City SC | Seattle Sounders | 0-2 |
| 10/21/23 8:00 PM | Portland Timbers | Houston Dynamo | 1-3 |
| 10/21/23 8:00 PM | San Jose Earthquakes | Austin FC | 1-1 |
| 10/21/23 8:00 PM | Los Angeles Galaxy | FC Dallas | 1-4 |
| 10/21/23 8:00 PM | Colorado Rapids | Real Salt Lake | 0-1 |
| 10/21/23 8:00 PM | Sporting Kansas City | Minnesota United FC | 3-1 |