| Date | Home | Away | Result |
|---|---|---|---|
| 9/30/23 6:30 PM | Toronto FC | Cincinnati FC | 2-3 |
| 9/30/23 6:30 PM | Orlando City | CF Montreal | 3-0 |
| 9/30/23 6:30 PM | New York Red Bulls | Chicago Fire | 0-1 |
| 9/30/23 6:30 PM | Inter Miami CF | New York City FC | 1-1 |
| 9/30/23 6:30 PM | New England Revolution | Charlotte FC | 2-1 |
| 9/30/23 6:30 PM | Columbus Crew | Philadelphia Union | 1-1 |
| 9/30/23 7:30 PM | Houston Dynamo | FC Dallas | 0-0 |
| 9/30/23 7:30 PM | Nashville SC | Seattle Sounders | 0-0 |
| 9/30/23 7:30 PM | Minnesota United FC | San Jose Earthquakes | 1-1 |
| 9/30/23 7:30 PM | St. Louis City SC | Sporting Kansas City | 4-1 |
| 9/30/23 8:30 PM | Colorado Rapids | Austin FC | 1-0 |
| 9/30/23 9:30 PM | Vancouver Whitecaps | D.C. United | 2-2 |
| 9/30/23 9:30 PM | Los Angeles Galaxy | Portland Timbers | 3-3 |
| 10/1/23 7:00 PM | Los Angeles FC | Real Salt Lake | 0-1 |