| Date | Home | Away | Result |
|---|---|---|---|
| 9/23/23 6:30 PM | Atlanta United FC | CF Montreal | 4-1 |
| 9/23/23 6:30 PM | D.C. United | New York Red Bulls | 3-5 |
| 9/23/23 6:30 PM | Philadelphia Union | Los Angeles FC | 0-0 |
| 9/23/23 6:30 PM | Cincinnati FC | Charlotte FC | 3-0 |
| 9/23/23 7:30 PM | Minnesota United FC | St. Louis City SC | 1-2 |
| 9/23/23 7:30 PM | FC Dallas | Columbus Crew | 1-1 |
| 9/23/23 7:30 PM | Sporting Kansas City | Houston Dynamo | 2-1 |
| 9/23/23 7:30 PM | Chicago Fire | New England Revolution | 2-2 |
| 9/23/23 8:30 PM | Real Salt Lake | Vancouver Whitecaps | 2-1 |
| 9/23/23 9:30 PM | San Jose Earthquakes | Nashville SC | 1-1 |
| 9/23/23 9:30 PM | Portland Timbers | Colorado Rapids | 3-2 |
| 9/24/23 12:00 PM | New York City FC | Toronto FC | 3-0 |
| 9/24/23 6:30 PM | Orlando City | Inter Miami CF | 1-1 |
| 9/24/23 8:30 PM | Austin FC | Los Angeles Galaxy | 3-3 |