| Date | Home | Away | Group | Result |
|---|---|---|---|---|
| 10/5/23 9:30 AM | FK Astana | Viktoria Plzen | Group C | 1-2 |
| 10/5/23 11:45 AM | Besiktas Istanbul | FC Lugano | Group D | 2-3 |
| 10/5/23 11:45 AM | FK Bodö/Glimt | Club Brugge | Group D | 0-1 |
| 10/5/23 11:45 AM | KF Ballkani Suhareka | Dinamo Zagreb | Group C | 2-0 |
| 10/5/23 11:45 AM | KAA Gent | Maccabi Tel Aviv | Group B | 2-0 |
| 10/5/23 11:45 AM | Breidablik Kopavogur | FC Zorya Lugansk | Group B | 0-1 |
| 10/5/23 11:45 AM | KÍ Klaksvík | OSC Lille | Group A | 0-0 |
| 10/5/23 11:45 AM | Olimpija Ljubljana | SK Slovan Bratislava | Group A | 0-1 |
| 10/5/23 2:00 PM | Fiorentina | Ferencvaros Budapest | Group F | 2-2 |
| 10/5/23 2:00 PM | FK Čukarički | KRC Genk | Group F | 0-2 |
| 10/5/23 2:00 PM | FC Nordsjaelland | PFC Ludogorets Razgrad | Group H | 7-1 |
| 10/5/23 2:00 PM | Spartak Trnava | Fenerbahce Istanbul | Group H | 1-2 |
| 10/5/23 2:00 PM | PAOK Saloniki | Eintracht Frankfurt | Group G | 2-1 |
| 10/5/23 2:00 PM | FC Aberdeen | HJK Helsinki | Group G | 1-1 |
| 10/5/23 2:00 PM | Aston Villa | HŠK Zrinjski Mostar | Group E | 1-0 |
| 10/5/23 2:00 PM | AZ Alkmaar | Legia Warschau | Group E | 1-0 |